Instruments
The spectrophotometer used in the method developed is a Genesys 10UV Scanning
ThermoSpectronic®
Spectrophotometer. The HPLC analysis was done using a PerkinElmer®
S200 HPLC System equipped with a quaternary pump, manual injector,
column oven, UV detector, and TotalChrom Workstation®
Software. The HPLC column
used was a 4.6-mm internal diameter, 250-mm length Brownlee Analytical PerkinElmer®
column with an octadecylsilane (C1-8) 5-µm particle size packing.