spectrophotometric assay, 1.48 mL of the ABTS+ solution and 20 lL
of the sample extract were mixed and measured immediately after
10 min at 734 nm at 30 C. All measurements were conducted in
triplicate, and the results were expressed as mg Trolox equivalents
(eq)/gram dry matter (Doblado et al., 2005). DPPH radical scavenging
activity was determined using the method originally developed
by Blois (1958). A portion (0.1 mL) of the extract solution was
mixed with 0.9 mL of a,a-diphenyl-b-picrylhydrazyl (DPPH) solution
(1.0 mM in methanol). The mixture was kept at ambient temperature
for 30 min prior to measurement of the absorbance at