In conclusion, the present study described the purification and
characterization of -amylase from a newly isolated B. methylotrophicus
strain P11-2. Some characters of the -amylase from B.
methylotrophicus strain P11-2 are similar to -amylases from other
strains of Bacillus spp., but most characters are different, such as
molecular weight, the end products of starch hydrolysis, the effects
of metal ions on the enzyme, and the first 10 amino acid residues
of the enzyme. Therefore, the -amylase produced by B. methylotrophicus
strain P11-2 should be a novel -amylase. It could be
attractive for practical applications in biotechnological processes