two combined genomes and their effects on gene expression
and phenotype, and of somatic hybrid meiotic
behavior and its implications on inheritance (e.g. disomic
vs. tetrasomic vs. intermediate), to devise efficient strategies
for triploid breeding based on complete understanding
of the genetic control and transmission of critical traits
from somatic hybrid parents to triploid offspring. Likewise,
such information is relevant to any long range breeding
strategies employed using sexual hybridization for breeding
at the tetraploid level in citrus.
Recently, there have been reports published that have
revealed interesting information on the base phenotypic
behavior of a selected citrus somatic hybrid, compared with
the donor parents and using RT–PCR to study gene
expression differences in key pathways. Bassene et al.
(2009a) reported on the components of fruit quality (sugars
and acids in the juice, and aromatic compounds found in
peel oil) in a somatic hybrid of Willowleaf mandarin
(Citrus deliciosa Ten.) ? Eureka lemon [Citrus limon (L.)
Burm.] and compared these to the diploid parents. They
observed that some characters, such as acids, were most
like those of the lemon parent, though fructose was closer
to mandarin, while sucrose and glucose levels were intermediate,
suggesting partial dominance of lemon for these
two combined genomes and their effects on gene expressionand phenotype, and of somatic hybrid meioticbehavior and its implications on inheritance (e.g. disomicvs. tetrasomic vs. intermediate), to devise efficient strategiesfor triploid breeding based on complete understandingof the genetic control and transmission of critical traitsfrom somatic hybrid parents to triploid offspring. Likewise,such information is relevant to any long range breedingstrategies employed using sexual hybridization for breedingat the tetraploid level in citrus.Recently, there have been reports published that haverevealed interesting information on the base phenotypicbehavior of a selected citrus somatic hybrid, compared withthe donor parents and using RT–PCR to study geneexpression differences in key pathways. Bassene et al.(2009a) reported on the components of fruit quality (sugarsand acids in the juice, and aromatic compounds found inpeel oil) in a somatic hybrid of Willowleaf mandarin(Citrus deliciosa Ten.) ? Eureka lemon [Citrus limon (L.)Burm.] and compared these to the diploid parents. Theyobserved that some characters, such as acids, were mostlike those of the lemon parent, though fructose was closerto mandarin, while sucrose and glucose levels were intermediate,suggesting partial dominance of lemon for these
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