the ischemic metabolites and the blood within the tissues except the experimental observation of the physiological behavior of the let-out blood; it never clots within the tissues same as the behavior of blood inside a blood donation bag due to presence of citrates inside the blood donation bag. Contrary to any other blood in the body like circulating blood or blood within the muscles caused by trauma, the blood trapped within the tissues of cupping therapy never clots inside the body, although it does not lack the ability to clot as it clots fast and hard when it is let outside the body. There should be a substance simulating citrates of the donation bag in effect which is being obliged to this trapped blood preventing its clotting within the tissues; that should be the acidic inflammatory metabolic mediators which are demonstrated at the end of each cupping procedure; they were isolated, tested and found to be mostly interferon, interleukin and tumor necrosis factor.