The HAA and LAA were measured using the trolox equivalent antioxidant capacity (TEAC) assay (Miller and Rice-Evans, 1997).Hydrophilic and lipophilic antioxidants were extracted from 0.3 g homogeneous suspension (three replicates) with 50% methanol or 50% acetone, respectively at 4 8C under constant shaking (300 rpm) for 12 h. Samples were centrifuged at 10,000 g for 7 min and each supernatant was recovered and used for antioxidant activity measurements. The antioxidant activity was measured at 734 nm in a Beckman DU 650 spectrophotometer. Two different calibration curves were constructed using freshly prepared trolox solutions for HAA and LAA determinations. The linear reading of the standard
curve was from 0 to 16 mM Trolox for HAA and LAA. Values were obtained from three replicates as mM Trolox/100 g fw.