The results of immunohistochemical staining with GPC-3 in hepatocellular lesions were recorded as negative (0–5% of tumor cells), focal positive (6–50% of tumor cells) and diffuse positive (450% of tumor cells) based on visual estimation of the entire tumor on the slide. The results with Hep Par 1 were similarly noted in poorly differentiated hepatocellular carcinomas and compared with GPC-3 staining. w2 test was used to compare the GPC-3 and Hep Par 1 results in poorly differentiated hepatocellular carcinoma. P-values of o0.05 were regarded as statistically significant