This work aimed at studying the effects of various culture mediaand carbon sources in order to formulate a general, simple and inexpensive medium to produce bacterial cellulose. More common carbon sources such as glucose, mannitol, sucrose, date syrup and foodgrade sucrose were used in this study. Carbon sources were compared in terms of biomass and production yield, and the morphology and structure of the bacterial cellulose obtained were studied. In this context, bacterial cellulose nanofibers produced in the selected media were characterized by means of xray diffraction (XRD), field emission scanning electron microscopy and thermogravimetric analysis (TGA).