Whole body vibration (WBV) has been proposed as an alternative exercise stimulus to produce adaptive responses similar to resistance exercise. Few studies have analysed acute hormonal responses to WBV. Purpose To evaluate neuromuscular and hormonal responses to an acute bout of isometric half-squat exercise with and without superimposition of WBV. Methods Seven healthy males (22.3+/-2.7 years) performed 10 sets of half squat isometric exercise for 1 min with 1-min rest between sets. Two separate trials were conducted either with WBV [30 Hz; 3.5 g (1 g=9.81 m.s2)] or without vibration (Control). Salivary concentration of testosterone and cortisol was collected and maximal isometric unilateral knee extensions (MVC) were completed before, immediately after, 1, 2 and 24 h after treatment. Results Significant decreases in MVC were observed immediately after (229.4+/-53.2 Nm), 1 h (231.6+/-59.9 Nm), and 2 h (233.0+/-59.1 Nm) after WBV compared with baseline (252.7+/-56.4 Nm; P