Device calibration
The analyzer is calibrated by registration of fluorescence of the aqueous extract complex solution with known content of zinc ions (0.0 mg/L and 1.0 mg/L). 5 mL of zinc solution are transferred into a 50 mL separation funnel, and 1mL of ascorbic acid solution (2 g/L), 5 mL of acetate buffer solution (pH 4.6–4.9), 1 mL of phenantroline solution (5 g/L) and 1 mL of sodium 8-mercapto-quinolinate solution (1 g/L) should be added. After two minutes, 5mL of chloroform are added and within one minute, zinc 8-mercapto-quinolinate should be extracted. After the layers separate, the lower layer may be filtered through the “red ribbon” filterdirectly to the cuvette of the FLUORAT®-02 analyzer. For analyzer calibration and all measurements light filter No 11 is used in the activation channel, and the light filter No 5 is used in the registration