The same immunoreagents (IL-3- and SCF-specific polyclonal antibodies and their
biotinylated forms) were used throughout the assays. The obtained data indicate that both
Nano-iPCR and iPCR are superior in sensitivity and detection range than ELISA. Furthermore,
Nano-iPCR is easier to perform than the other two methods. Nano-iPCR was used for
monitoring changes in concentration of free SCF during growth of mast cells in SCF-conditioned
media.