Temperature Effects on @H-II Release in Response to P&utile Administration of sQnRH-A The GtH-II release to 2 min pulsatile administration of 1 nM sGnRH-A was significantly increased at 20°C com- pared to 10°C and 15°C (Fig. 2). However, at 25°C the GtH-II response to 1 nM sGnRH-A was decreased and not statistically significant compared to at 2O”C, resulting in no significant differences in the GtH-II response at 25°C com- pared to at lO”C, 15°C and 20°C. At the doses of 100 nM and 10 nM, the GtH-II release responses to sGnRH-A at sGnRH-A (nM) FIG. 2. Temperature (10, 15,20 and 25’C) effects on GtH- II release from perifused pituitary fragments of common carp in response to 2-min pulses of three concentrations ( 1, 10 and 100 nM) of sGnRH-A. The GtH-II responses were quantified and expressed as percentage of prepulse. Each value represents mean -C SE (n = 4) from 4 columns of two separate experiments. Statistical significance was deter- mined by analysis of variance and Duncan’s multiple range test (p < 0.05); values that are not significantly different have a common underlining.