Although cryopreserved boar semen has been available since 1975, a major breakthrough in
commercial application has not yet occurred due to the high susceptibility of boar spermatozoa
to damage during cryopreservation and the complicated process required for deep
freezing. In recent years, the application of antioxidants during the cryopreservation of
boar semen has been the subject of considerable research aimed at improving the quality
of post-thaw semen. Centrifugation is necessary before using cryopreservation protocols for
freezing boar spermatozoa. Studies of the effect of different centrifugation regimens on boar
sperm recovery, yield and cryosurvival have made significant contributions. Therefore this
review elucidates results of recent applications of various antioxidants and centrifugation
regimens used in efforts to improve cryopreservation of boar spermatozoa. This review
is intended to enhance understanding of the roles of these antioxidants and centrifugation
regimens with respect to mechanisms that increase resistance to cryodamage of boar
spermatozoa. In addition, the discussion addresses the need for developing an objective
evaluation of effectiveness and estimating the prospect of application of new techniques
for the cryopreservation of boar semen and its use in artificial insemination.