Strains JU4-57 and BY4-523 were grown on YPD agar with staining and observed under microscopy
(10 x 100) as shown in Fig. 2. Primers used for the amplification of the D1/D2 fragment yield a fragment
about 542 and 623 bp for the JU4-57 and BY4-523 strains, respectively. The sequence obtained were
compared with those available in the GenBank, and the results showed high sequence similarity (99%)
with the type strain of Trichosporonoides spathulata for JU4-57 and Kodamaea ohmeri for BY4-523 base
on 23S rDNA. Therefore, JU4-57 and BY4-523 strains belonged to Trichosporonoides spathulata and
Kodamaea ohmeri, respectively.