Isolation of colonies
From each TSA culture, the colonies were transferred to the surface of the Cetrimide (Difco) in plates and incubated at 30–35°C for 18–24 h. The developed colonies were then submitted to oxidase and indol tests, followed by biochemical genera and species identification tests. In every plate, all the developed colonies were isolated for identification. The criteria used were colony morphology and colony color. The total number of colonies found at each sampling point (CFU/100 mL) and picked for identification, are shown in Tables 01 &2.