L3 larvae of Anisakis type 1 were collected by dissecting Micromesistius
poutassou (blue whiting), selecting only larvae with a
length > 2.0 cm. Larvae were axenised in antibiotic solution (Iglesias
et al. 1997), introduced into polystyrene plate wells with 2 ml
of sterile solution of 0.9% NaCl and 20 l of each dilution of test
compound, and then incubated at 36 .C in 5% CO2 atmosphere. As
controls, larvae were assayed without test compound under identical
experimental conditions. The final concentrations of the tested
compounds were: 125 g/ml; 65 g/ml, and 32.5 g/ml. Each dilution
was tested three times on larvae from fish captured on different
days.