Preparation of AgNPs was carried out according to our previous reported method (Barani et al. 2010). Nano liposome was prepared by heating method. The prepared lecithin solution (200 ppm, 50 ml) was stirred vigorously (approximately 1500 rpm) in a special designed bottle for 30 min at 70C and then cooled to room temperature.
Then,the reducing agent with an amount of twice the concentration of silver nitrate solution was added, while stirring on a hotplate stirrer for 15 min. The mixture of reducing agent and liposome colloidal solution was cooled to 0C, because the reducing agent is only activated by the reaction of silver salt according to Equation (1), and not by heating