Postprandial hypertriglyceridemia in mice. C57/BL
male mice weighing about 21–22 g were deprived of
food overnight before the experiments, and were divided
randomly into three groups (n ¼ 26 per group). The
mice were orally administered OTPP as a suspension
in 20% ethanol (500 or 1,000 mg/10 ml/kg) immediately
before administration of olive oil (5 ml/kg). Subgroups
of five or six mice were sequentially killed by
cervical dislocation at 0, 1.5, 3, 4, and 5 h after administration.
Blood was immediately taken by cardiac puncture
and collected in a heparinized tube. The triglyceride
level of plasma prepared by centrifugation was measured
using an enzyme assay kit, as described above.