In this section, the optimum pH and temperature for the working condition of purified CMCase which allow to obtain the highest amount of reducing sugars from hydrolysis reaction were studied. To find the optimum pH of purified CMCase, the reactions (containing 2% w/v of CMC) were set up at 50°C. The optimum pH of purified cellulase of MSL2 was pH 6.0 and the enzymatic hydrolysis efficiency retained more than 80% over the range of pH 4.0–7.0