Isolation of yeasts and moulds and preparation of inocula
The isolates of yeasts and moulds used in this work (Table 1)
were obtained from different cultivars of breba and fig (F. carica L.)
stored in equilibrium-modified atmosphere packaging (EMAP)
(Villalobos et al., 2014), using potato dextrose agar (PDA; Oxoid,
Unipath, Basingstoke, UK) acidified at pH 3.5 with a sterilised solution
of tartaric acid (10%) and Rose Bengal chloramphenicol agar
(RBCA; Oxoid). Yeasts were isolated from fruit before storage (day
0) or at day 7 of EMAP storage, while the moulds were isolated at
day 14 or 21 of EMAP storage when fungal decay was clearly present.
Two common pathogenic mould strains were also included in
the study: B. cinerea CECT20518 from the Spanish Type Culture
Collection, and M. laxa CA1, from the Department of Plant Health
(Government of Extremadura).