(Demetriades, Coupland, & McClements, 1997). Evidence for aggregation
and coalescence of emulsion oil droplets was obtained
via the Mastersizer with and without prior ultrasonic treatment for
30 s. Samples for the long-term stability tests had 0.05% (w/v) sodium
azide added as an antimicrobial agent. Freeze-thaw stability
of the emulsions was examined after 1 week storage in a freezer
at 18 C. The frozen emulsions were thawed in a water bath at
40 C for 30 min and then visually observed. Destabilized free oil
was determined as described earlier (Palanuwech, Potineni,
Roberts, & Coupland, 2003).