Each inoculum was prepared by inoculating the slant culture into 25 mL of the sterile liquid medium in 100 mL conical flask and growing it on a rotary shaker (100 rpm) for 48 h.
Each inoculum was prepared by inoculating the slant culture into 25 mL of the sterile liquid mediumin 100 mL conical flask and growing it on a rotary shaker (100 rpm) for 48 h.