In the second technique another PBS suspension was sonicated at a frequency of 40 KHz for 4 min in an ultrasonic cleaning bath (TESTLAB --- TB10TA, Argentina) to displace microorganisms
In the second technique another PBS suspension wassonicated at a frequency of 40 KHz for 4 min in an ultrasoniccleaning bath (TESTLAB --- TB10TA, Argentina) to displacemicroorganisms