The marine-derived A. terreus, Aspergillus versicolor, Aspergillus parasiticus and Penicillium brevicompactum, previously isolated from decayed wood samples collected from Ismalia, Egypt, were screened for inulinase production in this study. Strains were identified in the National Research Centre, Microbial Culture Collection Unit (MCCU) based on the morphological characterization according to its colonial and microscopic properties comparing with fungal species described by The strains were maintained on malt extract agar (MEA) medium at 4 ◦ C. The medium contained the following components (g/L): biomalt 20, agar 15, 800 ml sterile sea water and 200 ml distilled water