In order to explore the application effect of PBL didactics in teaching microbiology experiment,forty-four students of special domain in bio-tech in the college were taken as the research object.They were divided into two groups of twenty-two students each.Three experiments were chosen as the contents of given lectures respectively using LBL and PBL didactics to carry out the experiment lectures.The results showed that the average score of students taught with PBL didactics was higher than the ones with LBL.SPSS software analysis indicated that the effect of PBL didactics significantly excelled LBL one,demonstrated that PBL didactics could apply in teaching microbiology experiment.