Yeast survival determination
After thawing, the number of viable yeasts was determined in
the each dough sample, using the direct plate count method (Phimolsiripol
et al., 2008). Logarithmic dilution counts were carried
after the dilution of 10 g of dough in 90 g of tryptone salt solution
(Biokar Diagnostics, France). The diluted suspension (0.1 ml) was
cultured on Petri dishes with 15 ml of OGA (Oxytetracycline Glucose
Agar) base (Biokar Diagnostics, France). The cells were incubated
at 25 C for 72 h. The developed CFUs were expressed per
gram of dough. Triplicates plates were prepared for each of three
dough samples per sweet dough (SY and DY).