indicating that H. uvarum is a better strain for producing medium-chain fatty acids and the mixed fermentation of H. uvarum with S. cerevisiae can increase the concentrations of medium-chain fatty acids in Macabeo wine. PS, CS and MS co-fermentation can produce more esters, and theOAVs of total esters were 24.4%, 14.1% and 13.2% higher than that of a monoculture of SC.