On the basis of those findings, new PEF experiments were
designed, but with some restrictions. A new series of experiments
was conducted with lower spore load (104 spores mL1) to simulate
a scenario that is closer to conditions in industry. Since PEF treatment
lasts only microseconds, the spore inactivation was tested at
a processing temperature of 55 C together with electric pulses. As
a consequence thereof, the temperature level was limited, allowing
a setup for a relatively gentle processing close to nonthermal
treatment intensities. Finally, 65 C was chosen as the maximum
processing temperature for PEF-thermal enhanced treatments to
ensure the safety and functionality of the PEF chamber. Another
new variable was to increase the number of pulses up to 240 pulses
(2.5 ms) using refrigeration cycles between each set of pulses and
recirculation of milk in the system.
On the basis of those findings, new PEF experiments weredesigned, but with some restrictions. A new series of experimentswas conducted with lower spore load (104 spores mL1) to simulatea scenario that is closer to conditions in industry. Since PEF treatmentlasts only microseconds, the spore inactivation was tested ata processing temperature of 55 C together with electric pulses. Asa consequence thereof, the temperature level was limited, allowinga setup for a relatively gentle processing close to nonthermaltreatment intensities. Finally, 65 C was chosen as the maximumprocessing temperature for PEF-thermal enhanced treatments toensure the safety and functionality of the PEF chamber. Anothernew variable was to increase the number of pulses up to 240 pulses(2.5 ms) using refrigeration cycles between each set of pulses andrecirculation of milk in the system.
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