On the other hand, two-dimensional difference gel electrophoresis
(2-DE DIGE)−MS was applied to investigate the impact of three different
slaughtering techniques (asphyxia in air, asphyxia in ice and spinal
cord severance) on the postmortem integrity of muscle tissue proteins
in European sea bass (Addis et al., 2012). The results showed that
slaughtering by spinal cord severance preserves protein integrity better
than death by asphyxia, either in ice or in air. In this sense, effects of
postmortem storage temperature (1 or 18 °C for 5 days) on sea bass
muscle were also examined by 2-DE DIGE−MS (Terova et al., 2011).
As expected, the greatest alterations were observed upon postmortem
storage at 18 °C, mainly a significant decrease in the abundance of nucleoside
diphosphate kinase and phosphoglycerate mutase.