Nitric oxide (NO), a gas previously considered to be a toxic
chemical, has been shown to be a messenger molecule that
mediates diverse signaling pathways in target cells. It plays a
significant role in many physiological and pathological processes
including neurotransmission, blood pressure regulation,
inhibition of platelet adhesion and activation, and immunological
responses, etc. [1–5]. In biological systems, NO is
synthesized from L-arginine (L-Arg) by a family of enzymes
generally referred to as nitric oxide synthases (NOSs) in the
presence of trace oxygen. Production of NO plays a role in
liver injury as an inhibitor or agonist of cell signaling events
[6]. It has also been observed that an excess concentration of
NO is related to some human diseases [7, 8]. Therefore,
quantitative measurements of NO at biologically relevant
concentrations are essential for providing extremely useful
information to further understand the biological roles of NO
in vivo.
Nitric oxide (NO), a gas previously considered to be a toxicchemical, has been shown to be a messenger molecule thatmediates diverse signaling pathways in target cells. It plays asignificant role in many physiological and pathological processesincluding neurotransmission, blood pressure regulation,inhibition of platelet adhesion and activation, and immunologicalresponses, etc. [1–5]. In biological systems, NO issynthesized from L-arginine (L-Arg) by a family of enzymesgenerally referred to as nitric oxide synthases (NOSs) in thepresence of trace oxygen. Production of NO plays a role inliver injury as an inhibitor or agonist of cell signaling events[6]. It has also been observed that an excess concentration ofNO is related to some human diseases [7, 8]. Therefore,quantitative measurements of NO at biologically relevantconcentrations are essential for providing extremely usefulinformation to further understand the biological roles of NOin vivo.
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