2.4. Identification of the phytase producing fungal isolate
The phytase producing fungal isolate was identified by microscopic examination, the mycelium and conidiophores were observed after cultured on concavity slide with PDA medium at 28 C and then colored by fungus staining solution (20.0 g phenol, 20.0 ml lactic acid , 40.0 ml glycerin, 20 ml distilled water and 0.05 g cotton blue).