several methodologies are now available to evaluate oxidative stress and antioxidant capacity. In this study,we investigated serum level of ROM and periodontal level of protein oxidation. However, measurements of serum ROM may not be a completely validated method. Therefore,additional measurements of antioxidant capacity for scavenging free radicals would improve the reliability of our data.
The current study has the other limitations. The dentifrice in experimental group contained not only Pycnogenol but also anti-inflammatory and anti-bacterial agents, and
it may be possible that Pycnogenol had indirect effects on anti-ageing by itself
in periodontal tissue. Further studies are required to clarify this issue. In addition, the present study examined the anti-ageing effects of topical application ofTomarina1 in
6-month- and 10-month-old rats, considering the approach for adults. Further studies are needed to examine anti-ageing effects in older rats using the same procedure.Furthermore, clinical trials to examine the effect ofanti-ageing on application of Tomarina1 to human periodontal tissue are required.
In conclusion, topical application of Tomarina1 might suppress the effects of ageing on gingival collagen degradation, by decreasing oxidative stress in Fischer 344 male rats.