2.4. Storage test of pork
Pork loin was cut into pieces of 5 x 5 cm and a thickness of approx. 2 cm using a clean bench. These samples were inoculated with 1.3 x 10³ cfu of E. coli DSM 498 and finally vacuum packed with the lacquered composite film of polyethylene and polyamide. For reference samples, inoculated meat pieces were packaged with the according non-lacquered composite film. The samples were stored at 8 °C up to 7 days. The count of E. coli was monitored as a function of time. The viable count of E. coli was determined by pour-plate method using violet red bile dextrose agar (VRBD) after 0, 1, 3 and 7 days of storage. Each inoculation and the colony count were done in triplicates.