2.4. Detection of a H. pylori-specific gene in paraffin-embedded
biopsy samples
DNA was extracted from paraffin-embedded gastric biopsy tissues
using DEXPAD (Takara BioCo. Shiga, Japan) and subjected to
PCR. The primers ureF1 (ATA TTA TGG AAG AAG CGA GAG C) and
ureR (ATG GAA GTG TGA GCC GAT TTG), corresponding to bases
2783e2804 and 3076e3096, respectively, of the ureA gene of
H. pylori amplified 314-bp fragments. For the second round of PCR
amplification, primers ureR and ureF2 (CAT GAA GTG GGT ATT GAA
GC; þ2893e2912) were used.