The specific contaminants leading to pollution in water include a wide spectrum of chemicals, pathogens, and physical changes such as elevated temperature and discoloration. While many of the chemicals and substances that are regulated may be naturally occurring (calcium, sodium, iron, manganese, etc.) the concentration is often the key in determining what is a natural component of water and what is a contaminant. High concentrations of naturally occurring substances can have negative impacts on aquatic flora and fauna.