The B. lactis population suffered a remarkable decrease from 0 to 6 h of assay for the two frozen storage periods evaluated
(30 d and 120 d). Moreover, the reduction was more evident after 4 h and 6 h of assay, which coincides with the enteric phase. For these 2 stages (4 h and 6 h), it was also observed that the storage time influenced the probiotic viability and the P120 samples reached lower bacterial counts when compared to P30 (p < 0.05).