S. varians was obtained from Kong ju National University
(Chungnam, Republic of Korea)and maintained in Bold’sbasal medium (BBM)according to the method of Bold (1949). The plant was irradiated with gamma rays(8kGy)to induce the formation of mutants,and plants that survived treatment were collected.Both S. varians wild-type(WT) and mutants(Mut)were subsequently cultured,and methanol extracts were prepared.