Twenty-five
seeds (either filled or unfilled grain) of each cultivar
selected at random were spaced on damp 9-cm Whatman
No. 1 filter paper in plastic Petri dishes and each one
was replicated 16 times. The plates were incubated during
12-h-light and 12-h-darkness cycles at 25 2 8C for 4 days
to detect the blast pathogen and this was continued up to
8 days for other fungi detection