Rice powders of three varieties were obtained by
milling rice grain in a local grinding mill and passed
through a 500 µm sieve screen. The powder samples
were extracted with 80% methanol (1:10 w/v) at
room temperature for 24 h. After filtration by filter
paper (Whatman no.4), the filtrates were evaporated
to remove the solvent by using rotary vacuum
evaporator. The residues were resuspended in water
and then freeze-dried. The dried extract powders
were obtained and stored at -20o
C before use.