Five bacterial stains were isolated from different untreated soils of agricultural, the stains were able to grow in a medium containing glyphosate as sole carbon or phosphorus source by enrichment cultures of these soils. Based on bacteria stain were identified though MALDI-TOF MS and biochemical properties . The bast strain amongst them was identified as Klebsiella pneumoniae. This isolate showed the highest growth level in the presence of glyphosate.