The effects of feeding p-carotene (BC) on levels ofBCand vitamin A (retinol) in blood and tissues, and on beef quality, were evaluated in 120 steers. Each steer received supplementary BC (at concentrations of 0, 600, 1200, or 1800 mg/day) for 90 days and then received no supplementary BC for 60 days. BC significantly increased in blood serum, liver, and subcutaneous and omental fat; linearly increased in the intestine and muscle; and remained unchanged in perirenal fat during supplementation. Differences between treatment groups were elim inated in subcutaneous and omental fat and in the liver by days 120 and 150, respectively, but remained signif- icant at day 150 in blood. Retinol increased significantly in the liver and intestine during supplementation. Intramuscular fat content, meat color, and retinol in blood, muscle, or adipose tissues were not affected. Backfat thickness decreased slightly with increasing BC supplementation and significantly differed between groups during depletion