A novel magnetically separable laccase immobilized system was constructed by adsorbing laccase into
bimodal carbon-based mesoporous magnetic composites (CMMC). A large adsorption capacity
(491.7 mg g1), excellent activity recovery (91.0%) and broader pH and temperature profiles than free
laccase have been exhibited by the immobilized laccase. Thermal stability was enhanced to a great extent
and operational stability was increased to a certain extent. The shift of kinetic parameters indicated affinity
change between enzyme and substrate. Application of the immobilized system in phenol and
p-chlorophenol removal was investigated in a batch system. Adsorption effects of the support were responsible
for the quick removal rate in the first hour, and up to 78% and 84% of phenol and p-chlorophenol were
removed in the end of the reaction, respectively, indicating that the magnetic bimodal mesoporous carbon
is a promising carrier for both immobilization of laccase and further application in phenol removal.