Cotton seed material
Cotton (Gossypium hirsutum L.) cv. Coker 312 seeds were washed with tap and double distilled water. The seeds were sterilized with HgCl2 (0.1%) followed by rinsing with sterile water. The seeds were inoculated in 1/2 MS medium (Murashighe and Skoog’s) and incubated for 2 weeks at 16 h photo period, 28 °C temperature for germination on culture racks.