2.2.3. Laboratory methods for detection of Salmonella in chicken and egg
samples
All food samples were tested in the Food and Environmental
Laboratory at the IMVS. Chicken meat, egg contents and the external
surface of eggs were tested forSalmonella.Cracked eggs were excluded
from all testing (external and contents). Food samples were prepared
in sterile buffered peptone water, incubated at 36 ±1 °C for 16–20 h.
Sub-samples (0.1 mL) of all incubated enrichment broths (sterile
buffered peptone water) were inoculated into 10 mL of RappaportVassiliadis (RV) Broth and incubated at 42 ±1 °C for 18–24 h. The
TECRA® Salmonella ULTIMA test kit is an ELISA (enzyme linked
immuno-sorbent assay) based test