The calves aged 90 days were randomly divided into four
groups of six calves each. All animals were inoculated intravenously
with 3.6 × 107 erythrocytes parasitized with UFMG2 isolate, a
highly virulent sample of A. marginale (Genbank nº EU676175)
causing high morbidity and mortality rates in susceptible cattle
using indirect fluorescent antibody test (IFAT) to ensure that the
study calves were not infected with hemoparasites.