Physiological and Biochemical
Characteristics
The ability of two actinomycetes strains in
utilizing various carbon compounds as
source of energy is mentioned in table 2.
Isolate I2 was capable of utilizing all the
carbon sources except arabinose whereas
isolate I1 could not utilize sucrose, raffinose,
fructose, rhamnose and arabinose.
Sodium chloride tolerance was observed
only at 5% concentration and no growth at
higher concentrations in both the isolates as
shown in table 2. Both the strains showed
pH tolerance at different ranges of pH 5–9
as shown in table 2. However, growth of
isolate I2 was not observed at pH 5.
The results of biochemical tests are
mentioned in table 3. Both the isolates were
positive for catalase test, cellulose
degradation, H 2 S production, starch
hydrolysis and caseinase activity. Isolate I1
was negative whereas isolate I2 was positive
for lypolytic activity, while both the isolates
were negative for gelatin liquefaction.
Based on phenotypic, physiological and
biochemical characteristics the two isolates
were identified to be Streptomyces spp., by
matching the results obtained with the keys
given for actinomycetes in ISP
(International Streptomyces Project).
Physiological and BiochemicalCharacteristicsThe ability of two actinomycetes strains inutilizing various carbon compounds assource of energy is mentioned in table 2.Isolate I2 was capable of utilizing all thecarbon sources except arabinose whereasisolate I1 could not utilize sucrose, raffinose,fructose, rhamnose and arabinose.Sodium chloride tolerance was observedonly at 5% concentration and no growth athigher concentrations in both the isolates asshown in table 2. Both the strains showedpH tolerance at different ranges of pH 5–9as shown in table 2. However, growth ofisolate I2 was not observed at pH 5.The results of biochemical tests arementioned in table 3. Both the isolates werepositive for catalase test, cellulosedegradation, H 2 S production, starchhydrolysis and caseinase activity. Isolate I1was negative whereas isolate I2 was positivefor lypolytic activity, while both the isolateswere negative for gelatin liquefaction.Based on phenotypic, physiological andbiochemical characteristics the two isolateswere identified to be Streptomyces spp., bymatching the results obtained with the keysgiven for actinomycetes in ISP(International Streptomyces Project).
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