Note: (a) V1 represents the volume of Cl-3 ืreagent consumed by enzyme solution.(b) V0 represents
the volume of Cl-3 reagent consumed by the initial water.(c) Ec represents the concentration of enzyme solution which should be within the range of 0.250.02%. (d)The number 372 indicated the
coefficient of volume conversion from Cl-3 reagent to enzyme solution in the formula