Live-dead
BacLight bacterial viability kit (L7007;
Molecular Probes Inc., Eugene, OR) was
used for assessing the percentage of live
and dead R. solanacearum cells after
treatment with the oils in a direct contact
assay. This method has also been used
previously for assessing viability of R.
solanacearum cells in pathogen survival
and disease management studies (5,24).