High Performance Liquid Chromatography (Waters C 18 column, USA) was performed for sample isolated by TLC. Sample was dissolved in HPLC grade methanol in concentration of about 1-10μg/ml and 20μl of the solution was injected in the column RP-C18 and analyzed by PDA detector. The wavelength range was 250 - 500nm. The mobile phase components acetonitrile: water was used in a gradient form, which varied with change in time. The sampling rate was kept 2 (points/sec), total flow rate was kept 0.70 ml/min, filter time constant was 1.0000 sec and the software installed was Empower 2 software build 2154 SPs. Service pack H DB ID: 908711544