An effort to overcome losses from leaf rust disease that has been done is through crop rotation, the use of pesticides, and planting resistant varieties. Suprapto (1992) suggested that the use of resistant varieties can reduce yield losses due to soybean rust pathogen attack. The use of resistant varieties is a best method, because it is more economical and not the Result environmental pollution. Soybean is an important crop because of its role as a source of vegetable protein and can be used as industrial raw materials. In Indonesia the result is still low so every year need to import large enough to meet national demand (Karamoy, 2009). The low productivity of soybean and high production costs caused soybean production in the country is still very difficult to keep pace with the increasing demands, but by looking at the potential production is still possible to be improved. Leaf rust disease caused by P. pachyrhizi often affects soybean plants is one of the causes of low production. The resistance of varieties is one important component of disease control and very beneficial in crop protection. The resistance of varieties is the nature of a population of plants that allow the population to withstand the attack of a kind that cause disease so that damage is not meant for production decline (Sudjono, 1979). Soybean varieties have different resistance. The resilience of one variety of soybean rust pathogen can vary depending on the location of the testing and the age of the plant (Semangun, 1990). Suyamto et al. (1990) reported that the resistant soybean varieties are generally dark green leafy, a bit old, and live longer than those susceptible. Known, each plant has a mechanical and chemical barrier different. According to Hartana (1986) mechanical barriers may be morphological factors that exist in every plant, could form the content of wax, cuticle thickness, leaf epidermis and stomata. Bonde et al. (1976); McLean (1979) and Sudjono et al. (1975) reported that the pathogen infection leaf rust on soybean plants not through stomata, or rarely directly to the stomata, but the infection sprouts uredospore directly penetrate the epidermal tissue, so that the wax layer and the epidermal tissue is thick will hinder or prevent the entry of fungi into plant tissue.